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cisplatin viability staining reagent  (fluidigm)


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    fluidigm cisplatin viability staining reagent
    Cisplatin Viability Staining Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 902 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cisplatin+viability+staining+reagent/Cell-ID+Cisplatin/pmc12322514-44-6-10
    Average 93 stars, based on 902 article reviews
    cisplatin viability staining reagent - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Clonal relationships between Tph and Tfh cells in patients with SLE and in murine lupus
    Article Snippet: .. 5 μM of cisplatin viability staining reagent (Fluidigm #201064) was added for two minutes and then diluted with culture media. .. After centrifugation, Human TruStain FcX Fc receptor blocking reagent (BioLegend #422302) was used at a 1:100 dilution in PBS with 2.5 g bovine serum albumin (Sigma Aldrich #A3059) and 100 mg of sodium azide (Sigma Aldrich #71289) for 10 minutes followed by incubation with conjugated surface antibodies for 30 minutes.

    Article Title: Phenotyping the immune response to trauma: a multiparametric systems immunology approach
    Article Snippet: All CyTOF stains were performed at room temperature in 96-well round-bottom polypropylene plates (Corning). .. After washing cells by centrifugation at 200g for 5 minutes, 5 μM cisplatin viability staining reagent (Fluidigm) was added for 5 minutes. .. After centrifugation, TruStain FcX Fc receptor blocking reagent (BioLegend) was added for 10 minutes.


    Article Title: Phenotypic changes in immune cells induced by granulocyte and monocyte adsorptive apheresis in patients with severe COVID ‐19: An ex vivo study
    Article Snippet: .. For all stains, cisplatin viability staining reagent (Fluidigm Sciences) was added to plated cells for 5 min before staining. ..

    Article Title: Temporal phenotyping of neutrophils in post-cardiac arrest syndrome and extracorporeal membrane oxygenation-assisted resuscitation: Aa pilot study.
    Article Snippet: .. Cisplatin viability staining reagent (Cell-IDTMCisplatin-198Pt; Fluidigm Sciences, South San Francisco, CA, USA) was added to the cells for 5 minutes and the cells were washed using centrifugation. .. Human TruStain FcX (BioLegend, San Diego, CA, USA) was added to the cells, and the mixture was incubated for 5 minutes.

    Article Title: Neutrophil Heterogeneity Identifies an Association of LAMP1 With Proliferative Lupus Nephritis
    Article Snippet: .. A total of 5 μM of cisplatin viability staining reagent (Fluidigm, catalog 201064) was added for 2 min and then diluted with culture media. .. After centrifugation, Human TruStain FcX Fc receptor blocking reagent (BioLegend, catalog 422302) was used at a 1:100 dilution in CSB (PBS with 2.5 g BSA [MilliporeSigma, catalog A3059] and 100 mg of sodium azide [MilliporeSigma, catalog 71289]) for 10 min, followed by incubation with conjugated surface antibodies (Table ) for 30 min. All antibodies were obtained from the Harvard Medical Area CyTOF Antibody Resource and Core.

    Article Title: High-dimensional immunophenotyping reveals immune cell aberrations in patients with undiagnosed inflammatory and autoimmune diseases
    Article Snippet: .. After centrifugation and aspiration of the media, cisplatin viability staining reagent (Fluidigm) at a 5 μM concentration was added for 2 minutes and then the samples were diluted with the culture media. .. The cells were centrifuged and then blocked for 10 minutes using Human TruStain FcX Fc receptor blocking reagent (BioLegend) diluted 1:100 in media containing bovine serum albumin (Sigma Aldrich) and sodium azide (Sigma Adrich).

    Article Title: Combination of cancer vaccine with CD122-biased IL-2/anti-IL-2 Ab complex shapes the stem-like effector NK and CD8 + T cells against tumor.
    Article Snippet: .. Mass cytometry staining and measurement Mononuclear cells from the spleen and BM of mice were stained using 1 μM cisplatin viability staining reagent (Fluidigm) for 3 min, washed using cell staining buffer, and incubated with antibodies (5 μg/mL) against CD16/32 (BioLegend) for 10 min at room temperature (RT). ..

    Centrifugation:

    Article Title: Phenotyping the immune response to trauma: a multiparametric systems immunology approach
    Article Snippet: All CyTOF stains were performed at room temperature in 96-well round-bottom polypropylene plates (Corning). .. After washing cells by centrifugation at 200g for 5 minutes, 5 μM cisplatin viability staining reagent (Fluidigm) was added for 5 minutes. .. After centrifugation, TruStain FcX Fc receptor blocking reagent (BioLegend) was added for 10 minutes.

    Article Title: Temporal phenotyping of neutrophils in post-cardiac arrest syndrome and extracorporeal membrane oxygenation-assisted resuscitation: Aa pilot study.
    Article Snippet: .. Cisplatin viability staining reagent (Cell-IDTMCisplatin-198Pt; Fluidigm Sciences, South San Francisco, CA, USA) was added to the cells for 5 minutes and the cells were washed using centrifugation. .. Human TruStain FcX (BioLegend, San Diego, CA, USA) was added to the cells, and the mixture was incubated for 5 minutes.

    Article Title: High-dimensional immunophenotyping reveals immune cell aberrations in patients with undiagnosed inflammatory and autoimmune diseases
    Article Snippet: .. After centrifugation and aspiration of the media, cisplatin viability staining reagent (Fluidigm) at a 5 μM concentration was added for 2 minutes and then the samples were diluted with the culture media. .. The cells were centrifuged and then blocked for 10 minutes using Human TruStain FcX Fc receptor blocking reagent (BioLegend) diluted 1:100 in media containing bovine serum albumin (Sigma Aldrich) and sodium azide (Sigma Adrich).

    Concentration Assay:

    Article Title: High-dimensional immunophenotyping reveals immune cell aberrations in patients with undiagnosed inflammatory and autoimmune diseases
    Article Snippet: .. After centrifugation and aspiration of the media, cisplatin viability staining reagent (Fluidigm) at a 5 μM concentration was added for 2 minutes and then the samples were diluted with the culture media. .. The cells were centrifuged and then blocked for 10 minutes using Human TruStain FcX Fc receptor blocking reagent (BioLegend) diluted 1:100 in media containing bovine serum albumin (Sigma Aldrich) and sodium azide (Sigma Adrich).

    Mass Cytometry:

    Article Title: Combination of cancer vaccine with CD122-biased IL-2/anti-IL-2 Ab complex shapes the stem-like effector NK and CD8 + T cells against tumor.
    Article Snippet: .. Mass cytometry staining and measurement Mononuclear cells from the spleen and BM of mice were stained using 1 μM cisplatin viability staining reagent (Fluidigm) for 3 min, washed using cell staining buffer, and incubated with antibodies (5 μg/mL) against CD16/32 (BioLegend) for 10 min at room temperature (RT). ..

    Incubation:

    Article Title: Combination of cancer vaccine with CD122-biased IL-2/anti-IL-2 Ab complex shapes the stem-like effector NK and CD8 + T cells against tumor.
    Article Snippet: .. Mass cytometry staining and measurement Mononuclear cells from the spleen and BM of mice were stained using 1 μM cisplatin viability staining reagent (Fluidigm) for 3 min, washed using cell staining buffer, and incubated with antibodies (5 μg/mL) against CD16/32 (BioLegend) for 10 min at room temperature (RT). ..



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